DNA Data File for Scorpion Stinger

LOCUS       SCRTOX2NA     368 bp ss-mRNA            INV       15-MAR-1990

DEFINITION  A.australis (scorpion) mammalian neurotoxin (AaH I) mRNA, complete

            cds.

ACCESSION   M27701 J05102

KEYWORDS    neurotoxin.

SOURCE      A.australis telson, cDNA to mRNA, clone pcD-633.

  ORGANISM  Androctonus australis

            Eukaryota; Animalia; Metazoa; Arthropoda; Chelicerata; Arachnida;

            Scorpiones; Buthidae.

REFERENCE   1  (bases 1 to 368)

  AUTHORS   Bougis,P.E., Rochat,H. and Smith,L.A.

  TITLE     Precursors of Androctonus australis scorpion neurotoxins:

            Structures of precursors, processing outcomes, and expression of a

            functional recombinant toxin II

  JOURNAL   J. Biol. Chem. 264, 19259-19265 (1989)

  STANDARD  full staff_entry

COMMENT     Draft entry and computer-readable sequence for [J. Biol. Chem. 264,

            19259-19265 (1989)] kindly submitted

            by P.E.Bougis, 09-SEP-1989.

FEATURES             Location/Qualifiers

     mRNA            <1. .368

                     /note="AaH I mRNA"

     CDS             51. .302

                     /note="neurotoxin AaH I precursor"

                     /codon_start=51

     sig_peptide     51. .107

                     /note="neurotoxin AaH I signal peptide"

                     /codon_start=51

     mat_peptide     108. .296

                     /note="neurotoxin AaH I"

                     /codon_start=108

BASE COUNT      115 a     70 c     72 g    111 t

ORIGIN



M27701  Length: 368  December 26, 1991  19:19  Type: N  Check: 9685  ..



       1  AACAATCTAT TTTGCCTGCT TTTCCAGAAA ATTCCGTAAA ACGCTTCAAA 



      51  ATGAATTATT TGGTAATGAT TAGTTTGGCA CTTCTCCTCA TGATAGGTGT 



     101  GGAGAGTAAA CGTGACGGTT ATATTGTCTA TCCCAATAAC TGTGTATACC 



     151  ATTGTGTTCC ACCATGCGAC GGTTTATGTA AGAAAAACGG TGGTTCGAGT 



     201  GGCTCTTGCT CTTTTTTAGT TCCATCCGGA CTTGCCTGCT GGTGCAAAGA 



     251  CTTGCCCGAT AACGTACCGA TTAAAGATAC ATCACGGAAA TGCACCCGCT 



     301  GATAAACCTG TAGAGTAAAA TCAGAAAGAA TGTATCCTAA AAATAACTGG 



     351  TAAATAAACA TAAGTAAT